Finding new components of the circadian molecular machinery

research7smallWe are currently developing screenings that will allow us to find new circadian components. We are particularly excited in the possibility of finding relationship between different steps of the timekeeping system (i.e. transcriptional and post-translational control of Clk proteins). For finding these interactions we are performing double interaction screenings using a big number of mutants of circadian clock genes, miRNAs, RNA binding proteins and protein kinases. We are assaying these putative interactions not only in "canonical" conditions but also in situations in which the circadian timekeeping system is forced (i.e. extreme temperatures and light-dark regimes). We have already obtained unexpected and exciting results! In addition to these genetic screenings, we are developing genome-wide approaches that will allow us to find genes that regulate and are regulated by the circadian system at the transcriptional, post-transcriptional and translational levels.